Bacterial Gene Expression Technologies

Name: Bacterial Gene Expression Technologies
Brand: LAP Lambert Academic Publishing
SKU: 9786202004787
Price: 45.61 EUR
Availability: InStock

Автор: Mahmoud Zaky, Merna Abou-Selima

Език:

Корици: С меки корици

Издател: LAP Lambert Academic Publishing

Наличност: Външен склад

Изпращаме след 5-8 дни

45.61 € 89.21 лв

Random integration of the IVET vector into the pathogen chromosome is performed by insertion-duplica...

Информация за книгата

Автор

Mahmoud Zaky, Merna Abou-Selima

Език

Английски език

Корици

Книга - С меки корици

Издадена

2017

страници

124

EAN

9786202004787

Enbook ID

18038012

Издател

LAP Lambert Academic Publishing

Теглоt

203

Размери

150 x 220 x 7

Пълно описание

Random integration of the IVET vector into the pathogen chromosome is performed by insertion-duplication mutagenesis to create a pool of recombinant pathogens (this means that the gene in which the vector has inserted by homologous recombination is not disrupted). Recombinants can be selected using antibiotic resistance, as an additional marker is also on the integrated IVET construct. Pooled clones are then inoculated into the mouse. The two main types of IVET promoter trap strategies are (a) the complementation of auxotrophic mutation and (b) the expression of antibiotic resistance. Only those bacteria that contain the selective marker fused to a gene that is transcriptionally active in the host are able to survive. After a suitable infection period, bacteria that express the marker are isolated from lung, liver, spleen, and mesenteric tissue.

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Bacterial Gene Expression Technologies

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