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Книга Cell-Free Gene Expression Ashty S. Karim

Cell-Free Gene Expression

Methods and Protocols

Автор: Ashty S. Karim, Michael C. Jewett
Език: Английски език
Корици: С меки корици
Издател: Springer, Berlin
Наличност: Външен склад
Изпращаме след 10-18 дни
150.03 € 293.43 лв
This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable ne...

Информация за книгата

Автор
Ashty S. Karim, Michael C. Jewett
Език
Английски език
Корици
Книга - С меки корици
Издадена
2023
страници
435
EAN
9781071620007
Enbook ID
42644638
Издател
Springer, Berlin
Теглоt
784
Размери
178 x 254

Пълно описание

This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high-throughput cell-free experimentation. The second section provides an array of applications for CFE systems, such as metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cell-Free Gene Expression: Methods and Protocols serves as an ideal guide for researchers seeking technical methods to current aspects of CFE and related applications.

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