Enzyme-Mediated Ligation Methods

Name: Enzyme-Mediated Ligation Methods
Brand: Humana Press Inc.
SKU: 9781493995455
Price: 165.02 EUR
Availability: InStock

Автор: Timo Nuijens, Marcel Schmidt

Език:

Корици: С твърди корици

Издател: Humana Press Inc.

Наличност: Външен склад

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165.02 € 322.76 лв

This volume discusses different enzyme-catalyzed ligation methodologies for a variety of different c...

Информация за книгата

Автор

Timo Nuijens, Marcel Schmidt

Език

Английски език

Корици

Книга - С твърди корици

Издадена

2019

страници

371

EAN

9781493995455

ISBN

1493995456

Enbook ID

22113095

Издател

Humana Press Inc.

Теглоt

943

Размери

178 x 254 x 26

Пълно описание

This volume discusses different enzyme-catalyzed ligation methodologies for a variety of different chemical transformations. This book wants readers to view enzymes as a powerful tool in both academic and industrial research. Chapters in this book cover topics such as sortase A-mediated generation of site-specifically conjugated antibody-drug conjugates; omniligase-catalyzed inter- and intramolecular ligation; ligation catalyzed by microbial transglutaminase; peptide cyclization mediated by cyanobactin macrocyclases, butelase 1 and sortase A; using BioID as a tool for protein proximity labeling in living cells; and inducible, selective labeling of proteins via enzymatic oxidation of tyrosine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Enzyme-Mediated Ligation Methods is a valuable resource for students and scientists from different disciplines who are interested in using enzymatic strategies to answer their research questions.

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