Lipid Biochemistry

Name: Lipid Biochemistry
Brand: Chapman and Hall
SKU: 9780412266201
Price: 52.72 EUR
Availability: InStock

An Introduction

Автор: J.L. Harwood, M.I. Gurr

Език:

Корици: С меки корици

Издател: Chapman and Hall

Наличност: Външен склад

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Lipids can usually be extracted easily from tissues by making use of their hydrophobic characteristi...

Информация за книгата

Автор

J.L. Harwood, M.I. Gurr

Език

Английски език

Корици

Книга - С меки корици

Издадена

1991

страници

406

EAN

9780412266201

ISBN

0412266202

Enbook ID

02268891

Издател

Chapman and Hall

Теглоt

635

Размери

155 x 235 x 23

Пълно описание

Lipids can usually be extracted easily from tissues by making use of their hydrophobic characteristics. However, such extractions yield a complex mixture of different lipid classes which have to be purified further for quantitative analysis. Moreover, the crude lipid extract will be contami nated by other hydrophobic molecules, e.g. by intrinsic membrane proteins. Of the various types of separation processes, thin layer and column chromatography are most useful for intact lipids. High performance liquid chromatography (HPLC) is also rapidly becoming more popular, especially for the fractionation of molecular species of a given lipid class. The most powerful tool for quantitation of the majority of lipids is gas liquid chromatography (GLC). The method is very sensitive and, if adapted with capillary columns, can provide information with regard to such subtle features as the position or configuration of substitutions along acyl chains. By coupling GLC or HPLC to a radioactivity detector, then the techniques are also very useful for metabolic measurements. Although research laboratories use generally sophisticated analytical methods such as GLC to analyse and quantify lipid samples, chemical derivatie:ations are often used in hospitals. For these methods, the lipid samples are derivatized to yield a product which can be measured simply and accurately-usually by colour. Thus, total triacylglycerol, cholesterol or phospholipid-phosphorus can be quantitated conveniently without bothering with the extra information of molecular species, etc. which might be determined by more thorough analyses. REFERENCES Christie, w.w. (1982) Lipid Analysis, 2nd edn, Pergamon Press, Oxford.

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Lipid Biochemistry

An Introduction

Информация за книгата

Пълно описание

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