Microautoradiography and Electron Probe Analysis

Name: Microautoradiography and Electron Probe Analysis
Brand: Springer-Verlag Berlin and Heidelberg GmbH & Co. KG
SKU: 9783540059509
Price: 105.02 EUR
Availability: InStock

Their Application to Plant Physiology

Автор: U. Lüttge

Език:

Корици: С меки корици

Издател: Springer-Verlag Berlin and Heidelberg GmbH & Co. KG

Наличност: Външен склад

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In biological literature, several definitions of quantitative autoradio graphy are given. The term i...

Информация за книгата

Автор

U. Lüttge

Език

Английски език

Корици

Книга - С меки корици

Издадена

2012

страници

242

EAN

9783540059509

ISBN

3540059504

Enbook ID

06805498

Издател

Springer-Verlag Berlin and Heidelberg GmbH & Co. KG

Теглоt

500

Размери

155 x 235 x 14

Пълно описание

In biological literature, several definitions of quantitative autoradio graphy are given. The term is defined as either the determination and com parison of the density of silver grains above various structures or under varying conditions, or the determination of absolute quantities of radio activity. In both these cases, photometric measurement serves for more rapid and more exact evaluation of grain densities than would be possible by visual counting of the grains. The equipment generally used for the photometric measurement of silver grains consists of a microscope, a photocell, an electronic amplifier system and a display unit. Grains can be made accessible to photometric evaluation by various kinds of microscopic illumination: 1. Substage bright-field illumination. 2. Substage dark-field illumination. 3. Incident dark-field illumination. 4. Vertical bright-field illumination. With all these types of illumination, the relationship between the luminous flux I absorbed by the film, scattered into the objective and reflected or diffracted, and the flux 10 which is not affected by the film is used as a measure of grain density. Since these are differential measurements, the light beam I transmitted by the film is in itself a measure of grain density if the luminous flux 10 incident on the grains is kept constant. This approach has been used in a large number of measuring setups.

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Microautoradiography and Electron Probe Analysis

Their Application to Plant Physiology

Информация за книгата

Пълно описание

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Клиенти, които купиха тази книга, купиха също