Oligonucleotide Labeling and Detection by Serrs

Name: Oligonucleotide Labeling and Detection by Serrs
Brand: Scholars' Press
SKU: 9783639512724
Price: 92.32 EUR
Availability: InStock

Автор: Ljiljana Fruk

Език:

Корици: С меки корици

Издател: Scholars' Press

Наличност: Налично при издателя, по поръчка

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92.32 € 180.55 лв

A number of methods for detecting specific DNA sequences have been used to provide data for diagnosi...

Информация за книгата

Автор

Ljiljana Fruk

Език

Английски език

Корици

Книга - С меки корици

Издадена

2013

страници

220

EAN

9783639512724

ISBN

3639512723

Enbook ID

06938560

Издател

Scholars' Press

Теглоt

327

Размери

152 x 229 x 13

Пълно описание

A number of methods for detecting specific DNA sequences have been used to provide data for diagnosis of diseases and examination of gene expression. The most favoured is fluorescence detection although the addition of fluorescent labels can be disadvantageous due to the complex and expensive labelling chemistry. In this work, a novel method of oligonucleotide labelling was devised and surface enhanced resonance Raman scattering (SERRS) used as a detection technique. A number of SERRS active dyes containing benzotriazole groups were prepared and attached to DNA using Diels Alder chemistry. Benzotriazole maleimide dye and fluorescein were used in the design of a novel class of biosensor named SERRS Beacons, in which both fluorescence and SERRS are utilized for detection of minute amounts of DNA in solution. Low detection limits, simplicity of both the labelling chemistry and SERRS surface preparation, makes SERRS an excellent technique for oligonucleotide detection with the possibility of use in the rapidly growing DNA microarray and proteomic area.

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