Английски език
This authoritative collection of basic and advanced protocols permits biotechnologists to use plants rather than single cell fermentation systems for expressing recombinant proteins. The protocols provide clear step-by-step instructions for the cloning, expression, and analysis of a wide variety of recombinant proteins in plants, and for the cloning of genes into a number of different plant species. The book also describes promising experimental techniques that will become increasingly important in the future, and includes techniques for the characterization of medically important proteins expressed in plants.In Recombinant Proteins from Plants, leading researchers from around the world present the latest molecular and classical approaches to the separation, analysis, and characterization of recombinant plant proteins. Focusing on the large-scale, cost-effective production of such proteins for use as specialist industrial or therapeutic biomolecules, these authoritative scientists also include promising experimental techniques that will become increasingly important in the future-such techniques as those for the efficient transformation of monocots with Agrobacterium tumefaciens, for optimizing the stability of recombinant proteins, and for exploiting the immunotherapeutic potential of plant-expressed proteins. §Recombinant Proteins from Plants provides a comprehensive and detailed handbook of essential techniques that will benefit both scientists new to the field and established laboratories. It will become the standard reference for even highly skilled researchers working in this most exciting and fast developing field of biology.
