Screening for Reverse Cholesterol Transport efficiency

Name: Screening for Reverse Cholesterol Transport efficiency
Brand: Scholars' Press
SKU: 9783639664034
Price: 62.17 EUR
Availability: InStock

Автор: bhaswati sengupta

Език:

Корици: С меки корици

Издател: Scholars' Press

Наличност: Налично при издателя, по поръчка

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62.17 € 121.60 лв

Generation of foam cells, an essential step for reverse cholesterol transport (RCT) studies, uses th...

Информация за книгата

Автор

bhaswati sengupta

Език

Английски език

Корици

Книга - С меки корици

Издадена

2014

страници

EAN

9783639664034

ISBN

3639664035

Enbook ID

07175453

Издател

Scholars' Press

Теглоt

150

Размери

152 x 229 x 6

Пълно описание

Generation of foam cells, an essential step for reverse cholesterol transport (RCT) studies, uses the technique of receptor dependent macrophage loading with radiolabeled acetylated Low Density Lipoprotein (Ac-LDL). In this study, we used the ability of a biologically relevant detergent molecule, Lysophosphatidylcholine (Lyso PtdCho), to form mixed micelles with cholesterol or cholesteryl ester (CE) to generate macrophage foam cells. Fluorescent or radiolabelled cholesterol / Lyso PtdCho mixed micelles were prepared and incubated with RAW 264.7 or mouse peritoneal macrophages. Results showed that such micelles were quite stable at 4°C and retained the solubilized cholesterol during one month storage. Macrophages incubated with cholesterol or CE (unlabeled, fluorescently labeled or radiolabeled) / Lyso PtdCho mixed micelles accumulated CE as documented by microscopy, lipid staining, labeled oleate incorporation, and by thin layer chromatography (TLC). Such foam cells unloaded cholesterol when incubated with high density lipoprotein (HDL) and not with oxidized HDL (Ox-HDL). We propose that stable cholesterol or CE / Lyso PtdCho micelles would offer advantages over existing methods. O

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Screening for Reverse Cholesterol Transport efficiency

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