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Книга The Ubiquitin Code Manuel Rodriguez

The Ubiquitin Code

Автор: Manuel Rodriguez, Rosa Barrio
Език: Английски език
Корици: С твърди корици
Издател: Springer, Berlin
Наличност: Външен склад
Изпращаме след 10-13 дни
185.70 € 363.20 лв
This volume details novel and popular approaches to decipher the Ubiquitin Code. Chapters guide read...

Информация за книгата

Автор
Manuel Rodriguez, Rosa Barrio
Език
Английски език
Корици
Книга - С твърди корици
Издадена
2022
страници
245
EAN
9781071628584
Enbook ID
41391834
Издател
Springer, Berlin
Теглоt
666
Размери
178 x 254 x 20

Пълно описание

This volume details novel and popular approaches to decipher the Ubiquitin Code. Chapters guide readers through ubiquitin chain complexity, methods to generate non-hydrolysable ubiquitin-NEDD8, a fully synthetic method for the linear synthesis of fluorescently labelled rhodamine LC3A and LC3B, a HECT ligase-mediated ubiquitylation in vitro, a RNAi-based screening to identify DUBs, a Yuh1 activity assay, a pull-down to isolate K48 and K63 chains using specific nanobodies, identification of NeDDylated protein based in a NEDD8R74K mutant, in vivo identification of NEDDylated proteins from liver diseases, UbL-ID method to identify ubiquitin-like modified proteins, a LC3/GABARAP capturing system, and methods to isolate peptides that are closely associated with proteasomes or trapped inside the core particle of proteasomes in eukaryotic cells, among others. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Ubiquitin Code aims to to be a useful practical guide to researches to help further their study in this field.

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