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Книга Two-Hybrid Systems Paul N. MacDonald

Two-Hybrid Systems

Methods and Protocols

Автор: Paul N. MacDonald
Език: Английски език
Корици: С меки корици
Издател: Humana Press Inc.
Наличност: Външен склад
Изпращаме след 5-8 дни
105.39 € 206.12 лв
Paul N. MacDonald has assembled a collection of powerful molecular tools for examining and character...

Информация за книгата

Автор
Paul N. MacDonald
Език
Английски език
Корици
Книга - С меки корици
Издадена
2001
страници
336
EAN
9780896038080
ISBN
0896038084
Enbook ID
01400854
Издател
Humana Press Inc.
Теглоt
535
Размери
152 x 229 x 234

Пълно описание

Paul N. MacDonald has assembled a collection of powerful molecular tools for examining and characterizing protein-protein, protein-DNA, and protein-RNA interactions. The techniques range from the most basic (introducing plasmids into yeasts, interaction assays, and recovering the plasmids from yeast), to the most advanced alternative strategies (involving one-hybrid, split two-hybrid, three-hybrid, membrane recruitment systems, and mammalian systems). Methods are also provided for dealing with the well-known problem of artifacts and false positives and for identifying the interacting partners in important biological systems, including the SMAD and nuclear receptor pathways. To ensure ready reproducibility and robust results, each technique is described in step-by-step detail by researchers who employ it regularly.

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