2-D Proteome Analysis Protocols

Name: 2-D Proteome Analysis Protocols
Brand: Humana Press Inc.
SKU: 9781617370601
Price: 177.31 EUR
Availability: InStock

Автор: Andrew J. Link

Език:

Корици: С меки корици

Издател: Humana Press Inc.

Наличност: Външен склад в ограничено количество

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177.31 € 346.80 лв

With the completion of sequencing projects and the advancement of a- lytical tools for protein ident...

Информация за книгата

Автор

Andrew J. Link

Език

Английски език

Корици

Книга - С меки корици

Издадена

2010

страници

601

EAN

9781617370601

ISBN

1617370606

Enbook ID

02717249

Издател

Humana Press Inc.

Теглоt

899

Размери

152 x 229 x 35

Пълно описание

With the completion of sequencing projects and the advancement of a- lytical tools for protein identification, proteomics the study of the expressed part of the genome has become a major region of the burgeoning field of functional genomics. High-resolution 2-D gels can reveal virtually all p- teins present in a cell or tissue at any given time, including posttranslationally modified proteins. Changes in the expression and structure of most cellular proteins caused by differentiation or external stimuli can be displayed and eventually identified using 2-D protein gels. 2-D Proteome Analysis Protocols covers all aspects of the use of 2-D protein electrophoresis for the analysis of biological problems. The contri- tors include many of the leaders in the fields of biochemistry and analytical chemistry who were instrumental in the development of high-resolution 2-D gels, immobilized pH gradients, computer analysis, and mass spectromet- based protein identification methodologies. This book is intended as a benchtop manual and guide both for novices to 2-D gels and for those aficionados who wish to try the newer techniques. Any group using protein biochemistry especially in the fields of molecular biology, biochemistry, microbiology, and cell biology should find this book eminently useful. 2-D Proteome Analysis Protocols takes the researcher through the c- plete process of working with 2-D protein gels from making the protein - tract to finally identifying the proteins of interest. It includes protocols for generating 2-D protein extracts from most of the standard model organisms, including bacteria, yeast, nematode, Drosophila, plants, mouse, and human.

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2-D Proteome Analysis Protocols

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